Mini-course on SDS-PAGE

Sodium dodecyl sulphate- polyacrylamide electrophoresis technique is used widely in biochemistry, pharmacology, immunology  and molecular biology labs. by learning this technique, we can separate any proteins mixtures.

this technique is mainly used for proteins separation.

In this course, We will learn everything about this technique, including preparation of gel and sample.

This course includes main points:

Introduction which includes Sodium dodecyl sulphate- polyacrylamide electrophoresis definition and history technique.

Properties of SDS- PAGE.

The procedure of this system which includes several steps to separate mixture of proteins and applications of this technique.

Finally we recognise the variants and alternatives of this technique.

The gel preparation, sample preparation, electrophoresis and finally gel staining and documentation.

All proteins are sorted by size at the end of the electrophoretic separation and can then be analysed using other methods such as protein staining and immunological detection such as the Western Blot.

I tried to collect all data about sodium dodecyl sulphate polyacrylamide gel electrophoresis technique in these videos in simple way and concentrated to collect much informations and steps  in less time.

After finishing this course you know everything about this technique and it become easy to perform it in your lab under scientific considerations.