• Post category:StudyBullet-3
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RStudio

What you will learn

Basics of R. By this course you can have good attachment with R.

How to import data and set the directory for data?

How to install packages from Bioconductor?

Analyze differential expression (DE) between the tissues.


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Perform quality control and normalization.

Description

You want to be a professional in the field of Bioinformatics by R. If you don’t know how to use R on data problems and what ways of its uses, then this course will be beneficial for you. R is an emerging part of Bioinformatics. There are many sources to learn R and R have a sharp learning curve and often students get fed up by some things which look complicated. You should do practice it’s for the best experience.

This course is actually introducing R and elaborating R for Biomedical data. After this course, you will be able to do a complete analysis of biomedical data. After this, you can read the code according to your needs.

In this course, you will learn analysis for differential gene expression by Affymetrix Microarray and how to use R and RStudio for Bioinformatics. Code of this course will help you to do analysis and Slides will help you in the understanding of Microarray analysis. You will be able to know the PCA, box plot graphs, histograms, and heat map.

In summary, this course has been designed for all skill levels and even if you have no programming or statistical background you will be successful in this course!

English
language

Content

Introduction
Introduction
Installations of R and RStudio
Installations of Packages
Installations of Packages
If Package is not installed
Set the Directory
How to check and create the directory
If folder is not available
Hoe to set directory and download the data
Background Information on the Data
Information stored in ArrayExpress
Bioconductor ExpressionSets
Expression Sets
AnnotatedDataFrame
Create Expression set
pData
Quality Control
Perform PCA analysis
Quality Report
Summarization
Background Information and Calibration
Summarization
Relative Data Expression data quality analysis
Compute the RLE
Plot the RLE
RMA calibration of the data
RMA calibration of the data
Quality Assessment of the calibrated data
Material of Microarray Affymetrix analysis
End of the course